Requires separate runs for:
- Acid Detergent Fiber (ADF) – residue after digestion in acid detergent – estimate of cellulose and lignin.
- Neutral Detergent Fiber (NDF) – residue after digestion in neutral detergent – estimate of cellulose, hemicellulose, and lignin
- Crude Fiber – organic residue remaining after digesting with H2SO4 and NaOH