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Faculty of Agricultural, Life & Environmental Sciences

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Category: Chromatography

Agilent 1200 HPLC with ABI 4000 QTrap MS

October 7, 2015May 8, 2018

The QTrap is a triple quadrupole/ linear ion trap mass spectrometer capable of ESI and APCI. It can perform full scan MS and MS/MS as well as MRM (multiple reaction monitoring). It can be used to identify and quantify compounds. Read More …

Chromatography, High Performance Liquid Chromatographs

Shimadzu LC-20 UPLC with diode array detector

September 4, 2015May 14, 2018

The UPLC is able to operate at higher pressures than an HPLC, which allows the use of smaller column particles and lower flow rates. It typically results in better resolution and shorter run times than HPLC. The diode array detector Read More …

Chromatography, High Performance Liquid Chromatographs

Varian Prostar HPLC with fluorescence detector

September 4, 2015May 14, 2018

The fluorescence detector is typically more sensitive than the UV-VIS detector. Some compounds, such as amino acids, must be derivatized to increase their fluorescence.

Chromatography, High Performance Liquid Chromatographs

Shimadzu LC-20 HPLC with evaporative light scattering detector (ELSD)

September 4, 2015May 14, 2018

The ELSD detects light scattered by sample particles as they pass through a flow cell. It is not limited to specific functional groups, as any compound can scatter the light.

Chromatography, High Performance Liquid Chromatographs

Shimadzu LC-20 HPLC with diode array detector

September 4, 2015May 14, 2018

The diode array detector is able to monitor multiple wavelengths in the UV-VIS spectrum, from 190-700 nm.

Chromatography, High Performance Liquid Chromatographs

Agilent 7890A GC/MS

September 4, 2015May 14, 2018

It is capable of both EI and CI for compounds up to ~ 1000 MW. In addition to quantitative analysis of known compounds, it can be used to identify unknown compounds through unique fragmentation and by comparison with databases. It Read More …

Chromatography, Gas Chromatographs

Bruker Scion 456-GC

September 4, 2015May 14, 2018

It has both PTV and PWOC injectors and two detectors: flame ionization detector (FID) and thermal conductivity detector (TCD). The PTV injector allows for temperature programming of the inlet, while the PWOC injector allows for the use of packed and Read More …

Chromatography, Gas Chromatographs

Varian 430-GC with split/splitless injector and flame ionization detector (FID)

September 4, 2015May 14, 2018

The flame ionization detector is excellent for analyzing hydrocarbons and has excellent detection limits. It cannot analyze permanent gases, because they do not contain carbon-hydrogen bonds. The split/splitless injector allows for small sample amounts to be injected, allowing for better Read More …

Chromatography, Gas Chromatographs

Varian 3400 GC with cool-on-column injector and flame ionization detector (FID)

September 4, 2015May 14, 2018

The cool-on-column injector ensures the entire sample injected ends up on the column. It can be temperature programmed, which allows for samples containing compounds with widely varying boiling points to be analyzed with less discrimination occurring during the injection.

Chromatography, Gas Chromatographs

Contact us

Please e-mail comments on lab safety matters or the labs web pages to

Dr. Urmila Basu 
Faculty Service Officer
AFNS Labs & Genomics Unit Manager
Phone: 780-492-8712;
Fax: 780-492-4265
Email: ubasu@ualberta.ca

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